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Utilizing methylglyoxal and D-lactate in urine to evaluate saikosaponin C treatment in mice with accelerated nephrotoxic serum nephritis.
Lin, Chia-Yu; Lee, Jen-Ai; Lin, Po-Yeh; Hua, Shih-Chun; Tsai, Pei-Yun; Chen, Bi-Li; Lin, Chia-En; Lee, Tzong-Huei; Chen, Shih-Ming.
Afiliação
  • Lin CY; School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
  • Lee JA; Department of Pharmacy, Taipei Medical University Hospital, Taipei, Taiwan.
  • Lin PY; School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
  • Hua SC; School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
  • Tsai PY; School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
  • Chen BL; Department of Pharmacy, Cathay General Hospital, Taipei, Taiwan.
  • Lin CE; School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
  • Lee TH; Department of Pharmacy, WanFang Hospital, Taipei, Taiwan.
  • Chen SM; School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
PLoS One ; 15(10): e0241053, 2020.
Article em En | MEDLINE | ID: mdl-33104740
ABSTRACT
The relationship between methylglyoxal (MGO) and D-lactate during saikosaponin C (SSC) treatment of mice with accelerated nephrotoxic serum (NTS) nephritis was investigated. NTS nephritis was induced by administration of anti-basement membrane antibodies to C57BL/6 mice and three dosages of SSC were administered for 14 days. Proteinuria, blood urea nitrogen, serum creatinine, renal histology, urinary MGO and d-lactate changes were examined. Compared to the NTS control group, the middle dosage (10 mg/kg/day) of SSC significantly alleviated the development of nephritis based on urine protein measurements (34.40 ± 6.85 vs. 17.33 ± 4.79 mg/day, p<0.05). Pathological observation of the glomerular basement membrane (GBM) revealed monocyte infiltration, hypertrophy, and crescents were alleviated, and injury scoring also showed improved efficacy for the middle dose of SSC during nephritis (7.92 ± 1.37 vs. 3.50 ± 1.14, p<0.05). Moreover, the significant decreases in urinary levels of MGO (24.71 ± 3.46 vs. 16.72 ± 2.36 µg/mg, p<0.05) and D-lactate (0.31 ± 0.04 vs. 0.23 ± 0.02 µmol/mg, p<0.05) were consistent with the biochemical and pathological examinations. This study demonstrates that MGO and D-lactate may reflect the extent of damage and the efficacy of SSC in NTS nephritis; further studies are required to enable clinical application.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácido Oleanólico / Aldeído Pirúvico / Saponinas / Ácido Láctico / Glomerulonefrite Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácido Oleanólico / Aldeído Pirúvico / Saponinas / Ácido Láctico / Glomerulonefrite Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article