Dental Calculus as a Potential Biosource for Human Papillomavirus Detection in Oral Squamous Cell Carcinoma.
Asian Pac J Cancer Prev
; 21(10): 3093-3097, 2020 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-33112572
ABSTRACT
OBJECTIVE:
The infection of human papillomaviruses (HPVs) plays a role in the development of oral squamous cell carcinoma (OSCC). A poor oral hygiene and dental calculus may cause the infection to persist. Therefore, this study aimed to assess whether this dental calculus could serve as a potential biosource in early detection of HPVs in patients with OSCC.METHODS:
DNA was isolated from the dental calculus of people diagnosed with OSCC, and MY09/11 primer set was used to detect the presence of HPV. The positive samples were further sequenced and aligned using megablast NCBI BLAST tool to identify the HPV genotype.RESULTS:
Electrophoresis examination showed that 4 of 14 samples collected (29%) had a clear single band, of which three had 97% to 99% similarity to a high-risk genotype HPV-58. Meanwhile, the other sample had 99% similarity to an unclassified papillomaviridae.CONCLUSION:
Dental calculus is a promising source of HPV in oral cavity and could be used as a biomarker for early detection.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
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DNA Viral
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Neoplasias Bucais
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Carcinoma de Células Escamosas
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Cálculos Dentários
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Infecções por Papillomavirus
Tipo de estudo:
Diagnostic_studies
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Observational_studies
/
Prognostic_studies
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Risk_factors_studies
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Screening_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article