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Parasite Circulating Cell-free DNA in the Blood of Alveolar Echinococcosis Patients as a Diagnostic and Treatment-Status Indicator.
Fan, Haining; Gai, Wei; Zhang, Lingqiang; Ma, Yanyan; Wang, Haijiu; Chen, Xiaoping; Dong, Jiahong; Zhang, Yan; Bao, Haihua; Zhou, Ying; Ren, Li; Cairang, Yangdan; Hou, Lizhao; Ren, Bin; Wang, Zhan; Wang, Zhixin; Song, Cuidan.
Afiliação
  • Fan H; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Gai W; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China, National Engineering Research Center for Beijing Biochip Technology, Beijing, China, CapitalBio Corporation, Beijing, China.
  • Zhang L; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Ma Y; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Wang H; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Chen X; Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology, Wuhan, China.
  • Dong J; Beijng Tsinghua Changgung Hospital, Belling, China.
  • Zhang Y; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China, National Engineering Research Center for Beijing Biochip Technology, Beijing, China, CapitalBio Corporation, Beijing, China.
  • Bao H; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Zhou Y; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Ren L; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Cairang Y; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Hou L; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Ren B; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Wang Z; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Wang Z; Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China.
  • Song C; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China, National Engineering Research Center for Beijing Biochip Technology, Beijing, China, CapitalBio Corporation, Beijing, China.
Clin Infect Dis ; 73(1): e246-e251, 2021 07 01.
Article em En | MEDLINE | ID: mdl-33146713
BACKGROUND: Alveolar echinococcosis (AE) is a serious parasitic disease caused by the larvae of Echinococcus multilocularis. It is the less common but substantially more deadly of the 2 major echinococcosis diseases that can occur globally but are concentrated in central Asia. METHODS: We analyzed parasite circulating cell-free DNA (cfDNA) in 149 plasma samples using a DNA sequencing-based method (105 AE, 16 cystic echinococcosis, 4 liver cancer, 4 gallstones, and 20 healthy volunteers). After identifying the Echinococcus-specific cfDNA (Em-cfDNA) sequences in the samples, we determined whether Em-cfDNA could be used for AE diagnosis and as a potential indicator of the effectiveness of surgical treatment. We also examined potential associations between Em-cfDNA levels and clinical features of AE patients. RESULTS: Our work demonstrates that varying reads of Em-cfDNA were detectable in the plasma of 100% of preoperative AE patients and that all of the non-AE patients and healthy volunteers were negative. Em-cfDNA has good sensitivity and specificity for the diagnosis of AE. We also found that Em-cfDNA levels apparently have reference value for evaluating the therapeutic efficacy of surgery interventions for AE lesions. Finally, our analysis revealed that Em-cfDNA levels can reflect meaningful information about lesion size in preoperative AE patients. CONCLUSIONS: We demonstrate that sequencing-based monitoring of Em-cfDNA can be used in the clinic as a powerful diagnostic indicator for AE. We also note that there is a strong potential for use of this liquid-biopsy method to monitor ongoing disease status in postintervention AE patients.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Parasitos / Echinococcus multilocularis / Equinococose / Ácidos Nucleicos Livres Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Parasitos / Echinococcus multilocularis / Equinococose / Ácidos Nucleicos Livres Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article