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AMPA Receptor Surface Expression Is Regulated by S-Nitrosylation of Thorase and Transnitrosylation of NSF.
Umanah, George K E; Ghasemi, Mehdi; Yin, Xiling; Chang, Melissa; Kim, Jin Wan; Zhang, Jianmin; Ma, Erica; Scarffe, Leslie A; Lee, Yun-Il; Chen, Rong; Tangella, Kavya; McNamara, Amy; Abalde-Atristain, Leire; Dar, Mohamad A; Bennett, Samuel; Cortes, Marisol; Andrabi, Shaida A; Doulias, Paschalis-Thomas; Ischiropoulos, Harry; Dawson, Ted M; Dawson, Valina L.
Afiliação
  • Umanah GKE; Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: gumanah1@jhmi.edu.
  • Ghasemi M; Department of Neurology, University of Massachusetts School of Medicine, Worcester, MA 01655, USA.
  • Yin X; Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Chang M; University of California, Irvine, School of Medicine, Irvine, CA 92697-3950, USA.
  • Kim JW; University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
  • Zhang J; Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Beijing 100005, China.
  • Ma E; Johns Hopkins University Krieger School of Arts and Sciences, Baltimore, MD 21205, USA.
  • Scarffe LA; Division of Neurology, University of British Columbia, Vancouver, BC V5Z 1M9, Canada.
  • Lee YI; Division of Biotechnology, Well Aging Research Center, DGIST, Daegu, Republic of Korea.
  • Chen R; Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Tangella K; Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • McNamara A; Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Abalde-Atristain L; Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA.
  • Dar MA; Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Bennett S; Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Cortes M; Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Andrabi SA; Department of Pharmacology and Toxicology, The University of Alabama at Birmingham, Birmingham, AL 35294, USA.
  • Doulias PT; Department of Pediatrics, Children's Hospital of Philadelphia Research Institute, The University of Pennsylvania, Philadelphia, PA 19104, USA.
  • Ischiropoulos H; Department of Pediatrics, Children's Hospital of Philadelphia Research Institute, The University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Pharmacology, The University of Pennsylvania, Philadelphia, PA 19104, USA.
  • Dawson TM; Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Neurology, University of Massachusetts Schoo
  • Dawson VL; Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Neurology, University of Massachusetts Schoo
Cell Rep ; 33(5): 108329, 2020 11 03.
Article em En | MEDLINE | ID: mdl-33147468
ABSTRACT
The regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking affects multiple brain functions, such as learning and memory. We have previously shown that Thorase plays an important role in the internalization of AMPARs from the synaptic membrane. Here, we show that N-methyl-d-aspartate receptor (NMDAR) activation leads to increased S-nitrosylation of Thorase and N-ethylmaleimide-sensitive factor (NSF). S-nitrosylation of Thorase stabilizes Thorase-AMPAR complexes and enhances the internalization of AMPAR and interaction with protein-interacting C kinase 1 (PICK1). S-nitrosylated NSF is dependent on the S-nitrosylation of Thorase via trans-nitrosylation, which modulates the surface insertion of AMPARs. In the presence of the S-nitrosylation-deficient C137L Thorase mutant, AMPAR trafficking, long-term potentiation, and long-term depression are impaired. Overall, our data suggest that both S-nitrosylation and interactions of Thorase and NSF/PICK1 are required to modulate AMPAR-mediated synaptic plasticity. This study provides critical information that elucidates the mechanism underlying Thorase and NSF-mediated trafficking of AMPAR complexes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Membrana Celular / Receptores de AMPA / Proteínas Sensíveis a N-Etilmaleimida / ATPases Associadas a Diversas Atividades Celulares Limite: Animals / Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Membrana Celular / Receptores de AMPA / Proteínas Sensíveis a N-Etilmaleimida / ATPases Associadas a Diversas Atividades Celulares Limite: Animals / Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article