Single-cell transcriptomics reveals the heterogeneity of the decidual endothelial cells that participate in labor onset.
Eur Rev Med Pharmacol Sci
; 24(20): 10359-10365, 2020 10.
Article
em En
| MEDLINE
| ID: mdl-33155267
ABSTRACT
OBJECTIVE:
To investigate the heterogeneity of decidual endothelial cells and their changes during delivery. PATIENTS ANDMETHODS:
Single-cell RNA sequencing was used to characterize the transcriptomes of decidual endothelial cells before and after the onset of labor.RESULTS:
Decidual endothelial cells (9748 cells) were divided into five subgroups with different functions according to differences in the transcriptome. The functions of cluster 5 were enriched in vascular development and response to growth factors. After the onset of labor, the activities of each cluster were different, including the interleukin 17 pathway and regulation of ERK1 and ERK2 cascade. The downregulated genes were related to scavenger receptor (cluster 5), which may reflect the process of endothelial activation. In terms of genetic changes, cluster 5 may be more actively involved in labor than the other clusters.CONCLUSIONS:
Peripartum decidual endothelial cells are heterogeneous and participate in labor to varying degrees. One of the five subtypes of endothelial cells may be more actively involved in labor onset. Our findings may enable the assessment of decidual endothelial cells and labor onset.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Início do Trabalho de Parto
/
Decídua
/
Células Endoteliais
/
Análise de Célula Única
Limite:
Female
/
Humans
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Pregnancy
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article