Autofluorescence-Free In Vivo Imaging Using Polymer-Stabilized Nd3+-Doped YAG Nanocrystals.
ACS Appl Mater Interfaces
; 12(46): 51273-51284, 2020 Nov 18.
Article
em En
| MEDLINE
| ID: mdl-33156603
ABSTRACT
Neodymium-doped yttrium aluminum garnet (YAGNd3+) has been widely developed during roughly the past 60 years and has been an outstanding fluorescent material. It has been considered as the gold standard among multipurpose solid-state lasers. Yet, the successful downsizing of this system into the nanoregimen has been elusive, so far. Indeed, the synthesis of a garnet structure at the nanoscale, with enough crystalline quality for optical applications, was found to be quite challenging. Here, we present an improved solvothermal synthesis method producing YAGNd3+ nanocrystals of remarkably good structural quality. Adequate surface functionalization using asymmetric double-hydrophilic block copolymers, constituted of a metal-binding block and a neutral water-soluble block, provides stabilized YAGNd3+ nanocrystals with long-term colloidal stability in aqueous suspensions. These newly stabilized nanoprobes offer spectroscopic quality (long lifetimes, narrow emission lines, and large Stokes shifts) close to that of bulk YAGNd3+. The narrow emission lines of YAGNd3+ nanocrystals are exploited by differential infrared fluorescence imaging, thus achieving an autofluorescence-free in vivo readout. In addition, nanothermometry measurements, based on the ratiometric fluorescence of the stabilized YAGNd3+ nanocrystals, are demonstrated. The progress here reported paves the way for the implementation of this new stabilized YAGNd3+ system in the preclinical arena.
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MEDLINE
Assunto principal:
Polímeros
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Ítrio
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Nanopartículas
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Alumínio
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Imagem Óptica
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Neodímio
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article