Real-time fluorometric monitoring of monophenolase activity using a matrix-matched calibration curve.

Du, Dong; Guo, Nihong; Zhang, Ling; Wu, Yuting; Shang, Qi; Liu, Wenbin

Du, Dong; Guo, Nihong; Zhang, Ling; Wu, Yuting; Shang, Qi; Liu, Wenbin.

Afiliação

Du D; Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.
Guo N; Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.
Zhang L; Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.
Wu Y; Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.
Shang Q; Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.
Liu W; Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China. wbliu@scu.edu.cn.

Anal Bioanal Chem ; 413(2): 635-647, 2021 Jan.

Article em En | MEDLINE | ID: mdl-33159571

Assuntos

Calibragem; Di-Hidroxifenilalanina/análise; Fluorometria/métodos; Oxirredutases/análise; Tirosina/análise; Domínio Catalítico; Técnicas de Química Analítica; Cromatografia Líquida de Alta Pressão; Cromatografia Líquida; Inibidores Enzimáticos/química; Cinética; Limite de Detecção; Monofenol Mono-Oxigenase/química; Oxirredução; Reprodutibilidade dos Testes; Fatores de Tempo

Palavras-chave

Fluorescence; Monophenolase; Real-time; Synthetic matrix; Tyrosine

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oxirredutases / Tirosina / Calibragem / Di-Hidroxifenilalanina / Fluorometria Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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