Reactivity of anti-HLA class I polymorphic monoclonal antibodies with normal human skin.
Hybridoma
; 6(5): 545-54, 1987 Oct.
Article
em En
| MEDLINE
| ID: mdl-3315966
ABSTRACT
In this work the reactivity of 16 monoclonal antibodies raised against different HLA class I specificities was tested with human skin of healthy donors of known HLA typing. By indirect immunofluorescence, six antibodies reacted strongly with keratinocytes carrying the corresponding alloantigens. The reactivity of 3 other antibodies which was weak or absent using indirect immunofluorescence, was enhanced by various amplification systems such as avidin-biotin-peroxidase method, biotin-streptavidin-fluorescein complex and especially preliminary trypsin treatment that revealed alloantigens masked in the epidermis. The immunostaining of 4 antibodies was negative regardless of the method used. Some of the antibodies we tested cross-reacted with cytoplasmic antigens of keratinocytes. This study has allowed to select a battery of monoclonal antibodies which can specifically detect alloantigens on keratinocytes and will be useful for the recognition the cell origin in allografting experiments.
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Base de dados:
MEDLINE
Assunto principal:
Pele
/
Antígenos HLA
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article