Development and validation of an improved 3-methyl-2-benzothiazolinone hydrazone method for quantitative determination of reducing sugar ends in chitooligosaccharides.
Food Chem
; 343: 128532, 2021 May 01.
Article
em En
| MEDLINE
| ID: mdl-33172752
ABSTRACT
An accurate and sensitive analytical method for detecting and quantifying reducing sugar ends (RSE) in chitooligosaccharides (COSs) is the key quality parameter for evaluating their structure-function relationship and potential applications. In this work, we develop and validate a novel colorimetric assay with high accuracy and precision for determining RSE content using 3-methyl-2-benzothiazolinone hydrazone (MBTH). Under optimal conditions, the stoichiometry is verified using mono-, di-, and tri- glucosamine hydrochlorides, and the dilution ratio does not interfere with the RSE content measured at 590 nm. The regression equation of glucosamine reveal a good linear relationship (R2 = 0.9999). The detection limit, quantification limit, mean relative standard deviation (RSD), and recovery are 2.28 µM, 9.11 µM, 1.90%, and 98.0%, respectively. The newly developed method is potentially useful for monitoring COS hydrolysis, number average molecular weight, and chitosanase activity.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Quitina
/
Benzotiazóis
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Açúcares
/
Hidrazonas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article