Photocleavable Surfactant-Enabled Extracellular Matrix Proteomics.
Anal Chem
; 92(24): 15693-15698, 2020 12 15.
Article
em En
| MEDLINE
| ID: mdl-33232116
ABSTRACT
The extracellular matrix (ECM) provides an architectural meshwork that surrounds and supports cells. The dysregulation of heavily post-translationally modified ECM proteins directly contributes to various diseases. Mass spectrometry (MS)-based proteomics is an ideal tool to identify ECM proteins and characterize their post-translational modifications, but ECM proteomics remains challenging owing to the extremely low solubility of the ECM. Herein, enabled by effective solubilization of ECM proteins using our recently developed photocleavable surfactant, Azo, we have developed a streamlined ECM proteomic strategy that allows fast tissue decellularization, efficient extraction and enrichment of ECM proteins, and rapid digestion prior to reversed-phase liquid chromatography (RPLC)-MS analysis. A total of 173 and 225 unique ECM proteins from mouse mammary tumors have been identified using 1D and 2D RPLC-MS/MS, respectively. Moreover, 87 (from 1DLC-MS/MS) and 229 (from 2DLC-MS/MS) post-translational modifications of ECM proteins, including glycosylation, phosphorylation, and hydroxylation, were identified and localized. This Azo-enabled ECM proteomics strategy will streamline the analysis of ECM proteins and promote the study of ECM biology.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tensoativos
/
Compostos Azo
/
Proteômica
/
Matriz Extracelular
/
Proteínas de Neoplasias
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article