Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting.
Methods Mol Biol
; 2236: 203-217, 2021.
Article
em En
| MEDLINE
| ID: mdl-33237550
ABSTRACT
Flow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
/
Fenômenos Magnéticos
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Citometria de Fluxo
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Neutrófilos
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article