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Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting.
Rambault, Marion; Borkute, Rachana; Doz-Deblauwe, Emilie; Le-Vern, Yves; Winter, Nathalie; Dorhoi, Anca; Remot, Aude.
Afiliação
  • Rambault M; INRAE, Univ Tours, ISP, Nouzilly, France.
  • Borkute R; Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
  • Doz-Deblauwe E; INRAE, Univ Tours, ISP, Nouzilly, France.
  • Le-Vern Y; INRAE, Univ Tours, ISP, Nouzilly, France.
  • Winter N; INRAE, Univ Tours, ISP, Nouzilly, France.
  • Dorhoi A; Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany. anca.dorhoi@fli.de.
  • Remot A; Faculty of Mathematics and Natural Sciences, University of Greifswald, Greifswald, Germany. anca.dorhoi@fli.de.
Methods Mol Biol ; 2236: 203-217, 2021.
Article em En | MEDLINE | ID: mdl-33237550
ABSTRACT
Flow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Separação Celular / Fenômenos Magnéticos / Citometria de Fluxo / Neutrófilos Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Separação Celular / Fenômenos Magnéticos / Citometria de Fluxo / Neutrófilos Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article