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Preventing contamination of PCR-based multiplex assays including the use of a dedicated biosafety cabinet.
Bouam, A; Vincent, J J; Drancourt, M; Raoult, D; Levy, P Y.
Afiliação
  • Bouam A; POCRAMé, Marseille, France.
  • Vincent JJ; IRD, MEPHI, IHU Méditerranée Infection, Aix-Marseille University, Marseille, France.
  • Drancourt M; IHU Méditerranée Infection, Marseille, France.
  • Raoult D; IRD, MEPHI, IHU Méditerranée Infection, Aix-Marseille University, Marseille, France.
  • Levy PY; IHU Méditerranée Infection, Marseille, France.
Lett Appl Microbiol ; 72(1): 98-103, 2021 Jan.
Article em En | MEDLINE | ID: mdl-33245575
ABSTRACT
We retrospectively investigated cases of false-positive diagnoses using the BIOFIRE® FilmArray® meningitis/encephalitis (ME) panel to measure the impact of using a dedicated biosafety cabinet combined with preventive measures to reduce the prevalence of false-positive diagnoses due to pre-analytical in-laboratory contamination. False-positive results were identified by reviewing clinical data, biological parameters and cytology results of cerebrospinal fluid (CSF) samples showing discrepant results between the FilmArray ME panel and routine PCR assays. A total of 327 CSF were analysed over 16 weeks in point-of-care (POC) A and B, over two 8-week periods, periods 1 and 2. The analysis yielded 30 (9·17%) detection of at least one pathogen including 21/30 (70%) viruses and 9/30 (30%) bacteria. During period 1, POC-A and POC-B manipulated CSF under a non-dedicated hood featuring laminar flow, whereas during period 2, CSFs were manipulated under a dedicated biosafety cabinet without any airflow in POC-A. During period 1, false positives were detected in 3/114 CSF (2·63%) in POC-A and 1/36 (2·77%) in POC-B (P = 0·97); during period 2, false positives were detected in 0/139 CSF (0%) in POC-A and 1/38 (2·63%) in POC-B (P = 0·23). All false positives were bacterial. The use of a dedicated cabinet without ventilation along with preventive measures during period 2 in POC-A significantly reduced the number of false-positive results (P = 0·05). Preventive measures described in this study can mitigate false positives when using PCR-based multiplex assays such as the BIOFIRE FilmArray ME Panel for the diagnosis of meningitis and other infectious diseases.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias / Vírus / Sistemas Automatizados de Assistência Junto ao Leito / Contenção de Riscos Biológicos / Encefalite / Meningite Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans / Male Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias / Vírus / Sistemas Automatizados de Assistência Junto ao Leito / Contenção de Riscos Biológicos / Encefalite / Meningite Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans / Male Idioma: En Ano de publicação: 2021 Tipo de documento: Article