Cas9 in Human Embryos: On Target but No Repair.
Cell
; 183(6): 1464-1466, 2020 12 10.
Article
em En
| MEDLINE
| ID: mdl-33306952
ABSTRACT
In this issue of Cell, Zuccaro and colleagues show that on-target Cas9-mediated double-strand breaks cause chromosome loss or mis-repair of the disease allele in > 90% of human embryos. End joining repair pathways dominate, causing small insertions or deletions, which raises serious questions about using double-strand breaks for "gene surgery".
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Quebras de DNA de Cadeia Dupla
/
Reparo do DNA por Junção de Extremidades
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article