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Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016).
Silva-Sales, Marcelle; Leal, Elcio; Milagres, Flavio Augusto de Pádua; Brustulin, Rafael; Morais, Vanessa Dos Santos; Marcatti, Roberta; Araújo, Emerson Luiz Lima; Witkin, Steven S; Deng, Xutao; Sabino, Ester Cerdeira; Delwart, Eric; Luchs, Adriana; Costa, Antonio Charlys da.
Afiliação
  • Silva-Sales M; Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Laboratório de Virologia e Cultivo Celular, Goiânia, Goiás, Brazil.
  • Leal E; Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, Pará, Brazil.
  • Milagres FAP; Universidade Federal do Tocantins, Palmas, Tocantins, Brazil.
  • Brustulin R; Laboratório Central de Saúde Pública do Estado de Tocantins, Palmas, Tocantins, Brazil.
  • Morais VDS; Universidade Federal do Tocantins, Palmas, Tocantins, Brazil.
  • Marcatti R; Laboratório Central de Saúde Pública do Estado de Tocantins, Palmas, Tocantins, Brazil.
  • Araújo ELL; Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo, Brazil.
  • Witkin SS; Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo, Brazil.
  • Deng X; Ministério da Saúde, Secretaria de Vigilância em Saúde, Departamento de Articulação Estratégica de Vigilância em Saúde, Coordenação Geral de Laboratórios de Saúde Pública, Brasília, Distrito Federal, Brazil.
  • Sabino EC; Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo, Brazil.
  • Delwart E; Weill Cornell Medicine, Department of Obstetrics and Gynecology, New York, New York, USA.
  • Luchs A; Vitalant Research Institute, San Francisco, California, USA.
  • Costa ACD; University of California San Francisco, Department of Laboratory Medicine, San Francisco, California, USA.
Article em En | MEDLINE | ID: mdl-33331517
Surveillance of Rotavirus A (RVA) throughout the national territory is important to establish a more complete epidemiological-molecular scenario of this virus circulation in Brazil. The aim of the present study was to investigate the genetic diversity of RVA strains circulating in Tocantins State (Northern Brazil) during six years of post-vaccination follow-up (2010-2016). A total of 248 stool samples were screened by next generation sequencing and 107 (43.1%) nearly full length RVA genome sequences were obtained; one sample was co-infected with two RVA strains (G2/G8P[4]). Six G and P genotypes combinations were detected: G12P[8] strains (78.6%), as well as the G3P[8] (9.3%) and G1P[8] (0.9%) were associated with a Wa-like genogroup backbone. All G2P[4] (5.6%) and G8P[4] (2.8%) strains, including the mixed G2/G8P[4] infection (0.9%) showed the DS-1-like genetic background. The two G12P[4] strains (1.9%) were associated with distinct genetic backbones: Wa-like and DS-1-like. The phylogenetic analysis revealed the circulation of lineages G1-I, G2-IV, G3-III, G8-I and G12-III, and P[4]-V and P[8]-III of the VP7 and VP4 genes, respectively. Conserved clustering pattern and low genetic diversity were observed regarding VP1-VP3 and VP6, as well as NSP1-5 segments. We identified the same RVA circulation pattern reported in other Brazilian regions in the period of 2010-2016, suggesting that rural and low-income areas may not have a different RVA genotypic distribution compared to other parts of the country. The unique presentation of whole-genome data of RVA strains detected in the Tocantins State provides a baseline for monitoring variations in the genetic composition of RVA in this area.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por Rotavirus / Genoma Viral / Rotavirus Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Humans País como assunto: America do sul / Brasil Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por Rotavirus / Genoma Viral / Rotavirus Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Humans País como assunto: America do sul / Brasil Idioma: En Ano de publicação: 2020 Tipo de documento: Article