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Salmonella enterica serovar Typhi H58 clone has been endemic in Zimbabwe from 2012 to 2019.
Mashe, Tapfumanei; Leekitcharoenphon, Pimlapas; Mtapuri-Zinyowera, Sekesai; Kingsley, Robert A; Robertson, V; Tarupiwa, Andrew; Kock, Marleen M; Makombe, Evidence P; Chaibva, Blessmore V; Manangazira, Portia; Phiri, Isaac; Nyadundu, Simon; Chigwena, Chriswell T; Mufoya, Last P; Thilliez, Gaetan; Midzi, Stanely; Mwamakamba, Lusubilo W; Hamblion, Esther L; Matheu, Jorge; Jensen, Jacob D; Aarestrup, Frank M; Hendriksen, Rene S; Ehlers, Marthie M.
Afiliação
  • Mashe T; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.
  • Leekitcharoenphon P; National Microbiology Reference Laboratory, Harare, Zimbabwe.
  • Mtapuri-Zinyowera S; Technical University of Denmark, National Food Institute, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens Genomics, FAO Reference Laboratory for Antimicrobial Resistance and European Union Reference Laboratory for Antimicrobial Resistance, Kgs. Lyngby, Denmark.
  • Kingsley RA; National Microbiology Reference Laboratory, Harare, Zimbabwe.
  • Robertson V; Quadram Institute Bioscience, Norwich, UK.
  • Tarupiwa A; University of East Anglia, Norwich, UK.
  • Kock MM; Medical Microbiology, University of Zimbabwe, Zimbabwe.
  • Makombe EP; National Microbiology Reference Laboratory, Harare, Zimbabwe.
  • Chaibva BV; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.
  • Manangazira P; National Health Laboratory Service, Tshwane Academic Divisions, Pretoria, South Africa.
  • Phiri I; Gweru Provincial Hospital, Ministry of Health and Child Care, Gweru, Zimbabwe.
  • Nyadundu S; Ministry of Health and Child Care, Directorate of Pharmacy, Zimbabwe.
  • Chigwena CT; Ministry of Health and Child Care, Epidemiology and Disease Control, Zimbabwe.
  • Mufoya LP; Ministry of Health and Child Care, Epidemiology and Disease Control, Zimbabwe.
  • Thilliez G; Provincial Medical Directorate Offices, Midlands Province, Ministry of Health and Child Care, Gweru, Zimbabwe.
  • Midzi S; Ministry of Health and Child Care, Epidemiology and Disease Control, Zimbabwe.
  • Mwamakamba LW; Médecins Sans Frontières, Harare, Zimbabwe.
  • Hamblion EL; Quadram Institute Bioscience, Norwich, UK.
  • Matheu J; World Health Organization, Harare, Zimbabwe.
  • Jensen JD; World Health Organization Regional Office for Africa, Brazzaville, Republic of Congo.
  • Aarestrup FM; World Health Organization Regional Office for Africa, Brazzaville, Republic of Congo.
  • Hendriksen RS; World Health Organization, Geneva, Switzerland.
  • Ehlers MM; Technical University of Denmark, National Food Institute, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens Genomics, FAO Reference Laboratory for Antimicrobial Resistance and European Union Reference Laboratory for Antimicrobial Resistance, Kgs. Lyngby, Denmark.
J Antimicrob Chemother ; 76(5): 1160-1167, 2021 04 13.
Article em En | MEDLINE | ID: mdl-33347558
ABSTRACT

BACKGROUND:

Typhoid fever, caused by S. enterica ser. Typhi, continues to be a substantial health burden in developing countries. Little is known of the genotypic diversity of S. enterica ser. Typhi in Zimbabwe, but this is key for understanding the emergence and spread of this pathogen and devising interventions for its control.

OBJECTIVES:

To report the molecular epidemiology of S. enterica ser. Typhi outbreak strains circulating from 2012 to 2019 in Zimbabwe, using comparative genomics.

METHODS:

A review of typhoid cases records from 2012 to 2019 in Zimbabwe was performed. The phylogenetic relationship of outbreak isolates from 2012 to 2019 and emergence of antibiotic resistance was investigated by whole-genome sequence analysis.

RESULTS:

A total 22 479 suspected typhoid cases, 760 confirmed cases were reported from 2012 to 2019 and 29 isolates were sequenced. The majority of the sequenced isolates were predicted to confer resistance to aminoglycosides, ß-lactams, phenicols, sulphonamides, tetracycline and fluoroquinolones (including qnrS detection). The qnrS1 gene was associated with an IncN (subtype PST3) plasmid in 79% of the isolates. Whole-genome SNP analysis, SNP-based haplotyping and resistance determinant analysis showed that 93% of the isolates belonged to a single clade represented by multidrug-resistant H58 lineage I (4.3.1.1), with a maximum pair-wise distance of 22 SNPs.

CONCLUSIONS:

This study has provided detailed genotypic characterization of the outbreak strain, identified as S. Typhi 4.3.1.1 (H58). The strain has reduced susceptibility to ciprofloxacin due to qnrS carried by an IncN (subtype PST3) plasmid resulting from ongoing evolution to full resistance.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Salmonella typhi / Farmacorresistência Bacteriana Múltipla Limite: Humans País como assunto: Africa Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Salmonella typhi / Farmacorresistência Bacteriana Múltipla Limite: Humans País como assunto: Africa Idioma: En Ano de publicação: 2021 Tipo de documento: Article