LncRNA COL1A2-AS1 promotes skin fibroblast apoptosis by repressing p-Smad3 and promoting ß-catenin expression.
Exp Dermatol
; 30(8): 1090-1098, 2021 08.
Article
em En
| MEDLINE
| ID: mdl-33354832
ABSTRACT
LncRNA COL1A2-AS1 has been demonstrated to inhibit fibroblast proliferation of hypertrophic scars. However, the function of COL1A2-AS1 in normal skin fibroblasts remains poorly studied. Here, we report that overexpression of COL1A2-AS1 promoted normal skin fibroblast apoptosis. On the basis of mRNA-seq data and gene set enrichment analysis plus Kyoto encyclopedia of genes and genomes pathway analysis, 16 upregulated and 125 downregulated mRNAs were found; TGF-ß, Wnt, and MAPK pathways were potentially involved. Western blot assay confirmed that overexpression of COL1A2-AS1 repressed p-Smad3 expression and promoted ß-catenin expression. Furthermore, COL1A2-AS1 overexpression combined with either TGF-ß1 or siRNA against ß-catenin reversed the upregulation of apoptosis in the COL1A2-AS1 overexpression group. In conclusion, our study revealed the roles of COL1A2-AS1 in normal skin fibroblast apoptosis, with COL1A2-AS1 functioning by repressing p-Smad3 expression and promoting ß-catenin expression.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Colágeno Tipo I
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Proteína Smad3
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Beta Catenina
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Fibroblastos
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RNA Longo não Codificante
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article