Direct Measurement of Cerebrospinal Fluid Production in Mice.
Cell Rep
; 33(12): 108524, 2020 12 22.
Article
em En
| MEDLINE
| ID: mdl-33357428
ABSTRACT
The emerging interest in brain fluid transport has prompted a need for techniques that provide an understanding of what factors regulate cerebrospinal fluid (CSF) production. Here, we describe a methodology for direct quantification of CSF production in awake mice. We measure CSF production by placing a catheter in a lateral ventricle, while physically blocking outflow from the 4th ventricle. Using this methodology, we show that CSF production increases during isoflurane anesthesia, and to a lesser extent with ketamine/xylazine anesthesia, relative to the awake state. Aged mice have reduced CSF production, which is even lower in aged mice overexpressing amyloid-ß. Unexpectedly, CSF production in young female mice is 30% higher than in age-matched males. Altogether, the present observations imply that a reduction in CSF production might contribute to the age-related risk of proteinopathies but that the rate of CSF production and glymphatic fluid transport are not directly linked.
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Base de dados:
MEDLINE
Assunto principal:
Líquido Cefalorraquidiano
/
Sistema Glinfático
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article