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A comparison of airway pressures for inflation fixation of developing mouse lungs for stereological analyses.
Pérez-Bravo, David; Myti, Despoina; Mizíková, Ivana; Pfeffer, Tilman; Surate Solaligue, David E; Nardiello, Claudio; Vadász, István; Herold, Susanne; Seeger, Werner; Ahlbrecht, Katrin; Morty, Rory E.
Afiliação
  • Pérez-Bravo D; Department of Lung Development and Remodelling, Max Planck Institute for Heart and Lung Research, member of the German Center for Lung Research (DZL), Parkstrasse 1, 60231, Bad Nauheim, Germany.
  • Myti D; Department of Internal Medicine (Pulmonology), University of Giessen and Marburg Lung Center (UGMLC), member of the German Center for Lung Research (DZL), Aulweg 123, 35394, Giessen, Germany.
  • Mizíková I; Department of Lung Development and Remodelling, Max Planck Institute for Heart and Lung Research, member of the German Center for Lung Research (DZL), Parkstrasse 1, 60231, Bad Nauheim, Germany.
  • Pfeffer T; Department of Internal Medicine (Pulmonology), University of Giessen and Marburg Lung Center (UGMLC), member of the German Center for Lung Research (DZL), Aulweg 123, 35394, Giessen, Germany.
  • Surate Solaligue DE; Department of Lung Development and Remodelling, Max Planck Institute for Heart and Lung Research, member of the German Center for Lung Research (DZL), Parkstrasse 1, 60231, Bad Nauheim, Germany.
  • Nardiello C; Department of Internal Medicine (Pulmonology), University of Giessen and Marburg Lung Center (UGMLC), member of the German Center for Lung Research (DZL), Aulweg 123, 35394, Giessen, Germany.
  • Vadász I; Regenerative Medicine Program, The Ottawa Hospital Research Institute, 501 Smyth (Box 511), Ottawa, ON, 1H 8L6, Canada.
  • Herold S; Department of Cellular and Molecular Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON, K1H 8M5, Canada.
  • Seeger W; Department of Lung Development and Remodelling, Max Planck Institute for Heart and Lung Research, member of the German Center for Lung Research (DZL), Parkstrasse 1, 60231, Bad Nauheim, Germany.
  • Ahlbrecht K; Department of Internal Medicine (Pulmonology), University of Giessen and Marburg Lung Center (UGMLC), member of the German Center for Lung Research (DZL), Aulweg 123, 35394, Giessen, Germany.
  • Morty RE; Centre for Paediatric and Adolescent Medicine, Heidelberg University Hospital, Im Neuenheimer Feld 430, 69120, Heidelberg, Germany.
Histochem Cell Biol ; 155(2): 203-214, 2021 Feb.
Article em En | MEDLINE | ID: mdl-33372249
ABSTRACT
The morphometric analysis of lung structure using the principles of stereology has emerged as a powerful tool to describe the structural changes in lung architecture that accompany the development of lung disease that is experimentally modelled in adult mice. These stereological principles are now being applied to the study of the evolution of the lung architecture over the course of prenatal and postnatal lung development in mouse neonates and adolescents. The immature lung is structurally and functionally distinct from the adult lung, and has a smaller volume than does the adult lung. These differences have raised concerns about whether the inflation fixation of neonatal mouse lungs with the airway pressure (Paw) used for the inflation fixation of adult mouse lungs may cause distortion of the neonatal mouse lung structure, leading to the generation of artefacts in subsequent analyses. The objective of this study was to examine the impact of a Paw of 10, 20 and 30 cmH2O on the estimation of lung volumes and stereologically assessed parameters that describe the lung structure in developing mouse lungs. The data presented demonstrate that low Paw (10 cmH2O) leads to heterogeneity in the unfolding of alveolar structures within the lungs, and that high Paw (30 cmH2O) leads to an overestimation of the lung volume, and thus, affects the estimation of volume-dependent parameters, such as total alveoli number and gas-exchange surface area. Thus, these data support the use of a Paw of 20 cmH2O for inflation fixation in morphometric studies on neonatal mouse lungs.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pressão / Pulmão Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pressão / Pulmão Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article