The calcium transporter ANNEXIN1 mediates cold-induced calcium signaling and freezing tolerance in plants.
EMBO J
; 40(2): e104559, 2021 01 15.
Article
em En
| MEDLINE
| ID: mdl-33372703
ABSTRACT
The transient elevation of cytosolic free calcium concentration ([Ca2+ ]cyt ) induced by cold stress is a well-established phenomenon; however, the underlying mechanism remains elusive. Here, we report that the Ca2+ -permeable transporter ANNEXIN1 (AtANN1) mediates cold-triggered Ca2+ influx and freezing tolerance in Arabidopsis thaliana. The loss of function of AtANN1 substantially impaired freezing tolerance, reducing the cold-induced [Ca2+ ]cyt increase and upregulation of the cold-responsive CBF and COR genes. Further analysis showed that the OST1/SnRK2.6 kinase interacted with and phosphorylated AtANN1, which consequently enhanced its Ca2+ transport activity, thereby potentiating Ca2+ signaling. Consistent with these results and freezing sensitivity of ost1 mutants, the cold-induced [Ca2+ ]cyt elevation in the ost1-3 mutant was reduced. Genetic analysis indicated that AtANN1 acts downstream of OST1 in responses to cold stress. Our data thus uncover a cascade linking OST1-AtANN1 to cold-induced Ca2+ signal generation, which activates the cold response and consequently enhances freezing tolerance in Arabidopsis.
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Base de dados:
MEDLINE
Assunto principal:
Cálcio
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Arabidopsis
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Sinalização do Cálcio
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Proteínas de Arabidopsis
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Resposta ao Choque Frio
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article