Strategic design and improvement of the internal electron transfer of heme b domain-fused glucose dehydrogenase for use in direct electron transfer-type glucose sensors.

Ito, Kohei; Okuda-Shimazaki, Junko; Kojima, Katsuhiro; Mori, Kazushige; Tsugawa, Wakako; Asano, Ryutaro; Ikebukuro, Kazunori; Sode, Koji

Ito, Kohei; Okuda-Shimazaki, Junko; Kojima, Katsuhiro; Mori, Kazushige; Tsugawa, Wakako; Asano, Ryutaro; Ikebukuro, Kazunori; Sode, Koji.

Afiliação

Ito K; Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Okuda-Shimazaki J; Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC27599, USA.
Kojima K; Ultizyme International Ltd., 3-9-5 2F, Taihei, Sumida, Tokyo, 130-0011, Japan.
Mori K; Ultizyme International Ltd., 3-9-5 2F, Taihei, Sumida, Tokyo, 130-0011, Japan.
Tsugawa W; Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Asano R; Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Ikebukuro K; Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Sode K; Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC27599, USA. Electronic address: ksode@email.unc.edu.

Biosens Bioelectron ; 176: 112911, 2021 Mar 15.

Article em En | MEDLINE | ID: mdl-33421758

Assuntos

Técnicas Biossensoriais; Glucose 1-Desidrogenase; Glicemia; Automonitorização da Glicemia; Transporte de Elétrons; Elétrons; Flavina-Adenina Dinucleotídeo/metabolismo; Glucose; Glucose 1-Desidrogenase/metabolismo; Heme; Saccharomycetales

Palavras-chave

Direct electron transfer; Docking simulation; Fusion protein; Glucose dehydrogenase; Surface electrostatics

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Glucose 1-Desidrogenase Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Glucose 1-Desidrogenase Idioma: En Ano de publicação: 2021 Tipo de documento: Article