Your browser doesn't support javascript.
loading
Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish.
Gravato, Carlos; Abe, Flávia Renata; de Oliveira, Danielle Palma; Soares, Amadeu M V M; Domingues, Inês.
Afiliação
  • Gravato C; Faculdade de Ciências & CESAM, Universidade de Lisboa, Campo Grande, Lisboa, Portugal. cagravato@fc.ul.pt.
  • Abe FR; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • de Oliveira DP; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • Soares AMVM; National Institute for Alternative Technologies ofremoval of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM), Unesp, Institute of Chemistry, Araraquara, Brazil.
  • Domingues I; Department of Biology & CESAM, Universidade de Aveiro, Campus de Santiago, Aveiro, Portugal.
Methods Mol Biol ; 2240: 119-124, 2021.
Article em En | MEDLINE | ID: mdl-33423231
ABSTRACT
Acetylcholinesterase (AChE) is a useful biomarker for organophosphate and carbamate pesticides exposure. The inhibition of this enzyme has been associated with neurotoxicity and alterations at higher levels of biological organization, such as behavior and development impairments. In this chapter, we describe the methodologies for analyses of AChE activity in pools of 96 h of embryos of zebrafish (Danio rerio) using a spectrophotometric method adapted to 96-well microtiter plates.
Assuntos
Palavras-chave
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Acetilcolinesterase / Peixe-Zebra / Proteínas de Peixe-Zebra Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Acetilcolinesterase / Peixe-Zebra / Proteínas de Peixe-Zebra Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article