De novo expression and antibacterial potential of four lactoferricin peptides in cell-free protein synthesis system.

El-Baky, Nawal Abd; Elkhawaga, Maie Ahmed; Abdelkhalek, Eman Shawky; Sharaf, Mona Mohammed; Redwan, Elrashdy Mustafa; Kholef, Hoda Reda.

Afiliação

El-Baky NA; Therapeutic and Protective Proteins Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg EL-Arab, 21934, Alexandria, Egypt.
Elkhawaga MA; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Abdelkhalek ES; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Sharaf MM; Therapeutic and Protective Proteins Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg EL-Arab, 21934, Alexandria, Egypt.
Redwan EM; Therapeutic and Protective Proteins Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg EL-Arab, 21934, Alexandria, Egypt.
Kholef HR; Therapeutic and Protective Proteins Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg EL-Arab, 21934, Alexandria, Egypt.

Biotechnol Rep (Amst) ; 29: e00583, 2021 Mar.

Article em En | MEDLINE | ID: mdl-33425692

Palavras-chave

3D, three dimensional structures; Antimicrobial; Bioactive peptides; CAMH, cation-adjusted Mueller-Hinton broth; CFPS, cell-free protein synthesis; ConLFcin, consensus lactoferricin; ELISA, enzyme-linked immunosorbent assay; HSV, herpes simplex virus; In vitro protein synthesis; LC50, concentration lethal to 50 % of the cells; LFcin, lactoferricin; Lactoferricin; Lactoferrin; Lf, lactoferrin; MIC, minimum inhibitory concentration; MICs, minimum inhibitory concentrations; MRSA, methicillin-resistant Staphylococcus aureus; PBMCs, peripheral blood mononuclear cells; SD, Shine-Dalgarno sequence; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; bLFcin, bovine lactoferricin; cLFcin, camel lactoferricin; cLf, camel lactoferrin; hLFcin, human lactoferricin; hLf, human lactoferrin; p-NPP, p-Nitrophenyl phosphate

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article