Label-free analysis of gingival crevicular fluid (GCF) by surface enhanced Raman scattering (SERS).
Analyst
; 146(4): 1464-1471, 2021 Feb 21.
Article
em En
| MEDLINE
| ID: mdl-33427826
ABSTRACT
Gingival crevicular fluid (GCF) is an interesting biofluid reflecting the physiological and pathological states of a single dental element. Due to this unique feature, in recent years, metabolomic analysis of GCF has gained attention as a biometric tool for the diagnosis and therapy of periodontal disease. Traditional methods are, however, too slow, cumbersome and expensive for a health-care routine. Surface enhanced Raman scattering (SERS) can offer rapid and label-free detailed molecular fingerprints that can be used for biofluid analysis. Here we report the first SERS characterization of GCF using an easy and quick sample preparation. The dominant features in the SERS spectrum of GCF are ascribed to very few metabolites, in particular to uric acid, hypoxanthine, glutathione and ergothioneine. Additionally, we succeeded in differentiating between the SERS signal of GCF collected from healthy volunteers and the one collected from patients with periodontal disease.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
/
Líquido do Sulco Gengival
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article