RPA complexes in Caenorhabditis elegans meiosis; unique roles in replication, meiotic recombination and apoptosis.
Nucleic Acids Res
; 49(4): 2005-2026, 2021 02 26.
Article
em En
| MEDLINE
| ID: mdl-33476370
ABSTRACT
Replication Protein A (RPA) is a critical complex that acts in replication and promotes homologous recombination by allowing recombinase recruitment to processed DSB ends. Most organisms possess three RPA subunits (RPA1, RPA2, RPA3) that form a trimeric complex critical for viability. The Caenorhabditis elegans genome encodes RPA-1, RPA-2 and an RPA-2 paralog RPA-4. In our analysis, we determined that RPA-2 is critical for germline replication and normal repair of meiotic DSBs. Interestingly, RPA-1 but not RPA-2 is essential for somatic replication, in contrast to other organisms that require both subunits. Six different hetero- and homodimeric complexes containing permutations of RPA-1, RPA-2 and RPA-4 can be detected in whole animal extracts. Our in vivo studies indicate that RPA-1/4 dimer is less abundant in the nucleus and its formation is inhibited by RPA-2. While RPA-4 does not participate in replication or recombination, we find that RPA-4 inhibits RAD-51 filament formation and promotes apoptosis of a subset of damaged nuclei. Altogether these findings point to sub-functionalization and antagonistic roles of RPA complexes in C. elegans.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
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Caenorhabditis elegans
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Apoptose
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Proteínas de Caenorhabditis elegans
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Replicação do DNA
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Proteína de Replicação A
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Meiose
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article