A Palette of Minimally Tagged Sucrose Analogues for Real-Time Raman Imaging of Intracellular Plant Metabolism.
Angew Chem Int Ed Engl
; 60(14): 7637-7642, 2021 03 29.
Article
em En
| MEDLINE
| ID: mdl-33491852
ABSTRACT
Sucrose is the main saccharide used for long-distance transport in plants and plays an essential role in energy metabolism; however, there are no analogues for real-time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small (≈50â
Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne-tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real-time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Proteínas de Plantas
/
Sacarose
/
Azidas
/
Cumarínicos
/
Células Vegetais
/
Indicadores e Reagentes
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article