Your browser doesn't support javascript.
loading
A new, unquenched intermediate of LHCII.
Li, Fei; Liu, Cheng; Streckaite, Simona; Yang, Chunhong; Xu, Pengqi; Llansola-Portoles, Manuel J; Ilioaia, Cristian; Pascal, Andrew A; Croce, Roberta; Robert, Bruno.
Afiliação
  • Li F; Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
  • Liu C; Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
  • Streckaite S; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France.
  • Yang C; Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
  • Xu P; Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, Amsterdam, The Netherlands.
  • Llansola-Portoles MJ; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France.
  • Ilioaia C; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France. Electronic address: cristian.ilioaia@cea.fr.
  • Pascal AA; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France.
  • Croce R; Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, Amsterdam, The Netherlands.
  • Robert B; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France. Electronic address: bruno.robert@cea.fr.
J Biol Chem ; 296: 100322, 2021.
Article em En | MEDLINE | ID: mdl-33493515
ABSTRACT
When plants are exposed to high-light conditions, the potentially harmful excess energy is dissipated as heat, a process called non-photochemical quenching. Efficient energy dissipation can also be induced in the major light-harvesting complex of photosystem II (LHCII) in vitro, by altering the structure and interactions of several bound cofactors. In both cases, the extent of quenching has been correlated with conformational changes (twisting) affecting two bound carotenoids, neoxanthin, and one of the two luteins (in site L1). This lutein is directly involved in the quenching process, whereas neoxanthin senses the overall change in state without playing a direct role in energy dissipation. Here we describe the isolation of an intermediate state of LHCII, using the detergent n-dodecyl-α-D-maltoside, which exhibits the twisting of neoxanthin (along with changes in chlorophyll-protein interactions), in the absence of the L1 change or corresponding quenching. We demonstrate that neoxanthin is actually a reporter of the LHCII environment-probably reflecting a large-scale conformational change in the protein-whereas the appearance of excitation energy quenching is concomitant with the configuration change of the L1 carotenoid only, reflecting changes on a smaller scale. This unquenched LHCII intermediate, described here for the first time, provides for a deeper understanding of the molecular mechanism of quenching.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteínas de Arabidopsis / Complexos de Proteínas Captadores de Luz / Complexo de Proteína do Fotossistema II Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteínas de Arabidopsis / Complexos de Proteínas Captadores de Luz / Complexo de Proteína do Fotossistema II Idioma: En Ano de publicação: 2021 Tipo de documento: Article