Ion mobility mass spectrometry in the omics era: Challenges and opportunities for metabolomics and lipidomics.
Mass Spectrom Rev
; 41(5): 722-765, 2022 09.
Article
em En
| MEDLINE
| ID: mdl-33522625
ABSTRACT
Researchers worldwide are taking advantage of novel, commercially available, technologies, such as ion mobility mass spectrometry (IM-MS), for metabolomics and lipidomics applications in a variety of fields including life, biomedical, and food sciences. IM-MS provides three main technical advantages over traditional LC-MS workflows. Firstly, in addition to mass, IM-MS allows collision cross-section values to be measured for metabolites and lipids, a physicochemical identifier related to the chemical shape of an analyte that increases the confidence of identification. Second, IM-MS increases peak capacity and the signal-to-noise, improving fingerprinting as well as quantification, and better defining the spatial localization of metabolites and lipids in biological and food samples. Third, IM-MS can be coupled with various fragmentation modes, adding new tools to improve structural characterization and molecular annotation. Here, we review the state-of-the-art in IM-MS technologies and approaches utilized to support metabolomics and lipidomics applications and we assess the challenges and opportunities in this growing field.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Mobilidade Iônica
/
Lipidômica
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article