Apoptotic effects of alisol B 23acetate on gastric cancer cells.
Mol Med Rep
; 23(4)2021 04.
Article
em En
| MEDLINE
| ID: mdl-33537833
ABSTRACT
Alisol B 23acetate (AB23A) is a natural triterpenoid isolated from Alismatis rhizoma, which exhibits a number of pharmacological activities. In the present study, AB23Ainduced anticancer efficacy was examined in AGS gastric cancer cells. Cell viability assay, cell cycle analysis, caspase activity assay, western blotting and reactive oxygen species (ROS) assay were used to investigate the anticancer effects of AB23A on AGS cells. AB23A reduced the viability of AGS cells, increased the subG1 cell fraction and depolarized the mitochondrial membrane. Notably, AB23Ainduced cell death was associated with downregulation of the Bcell lymphoma 2 and survivin proteins, and upregulation of the Bax protein. In addition, AB23A increased caspase3 and 9 activities, and regulated the activation of mitogenactivated protein kinases (MAPK). Moreover, AB23A increased the production of reactive oxygen species. These results suggested that AB23A may induce apoptosis through cell cycle arrest and the mitochondrial pathway, accompanied by the caspase and MAPK signaling cascades. In conclusion, AB23A may have potential as a novel anticancer drug for the treatment of gastric cancer.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
/
Colestenonas
/
Apoptose
/
Sistema de Sinalização das MAP Quinases
/
Proteínas de Neoplasias
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article