Intestinal and hepatic effects of iron oxide nanoparticles.
Arch Toxicol
; 95(3): 895-905, 2021 03.
Article
em En
| MEDLINE
| ID: mdl-33554279
ABSTRACT
Iron oxide nanoparticles gain increasing attention due to their broad industrial use. However, safety concerns exist since their effects on human cells are still under investigation. The presence of iron oxide nanoparticles in the food pigment E172 has been shown recently. Here, we studied four iron oxide nanoparticles, one food pigment E172 and the ionic control FeSO4 regarding dissolution in biological media, uptake and transport, and cellular effects in vitro in human intestinal Caco-2 and HepaRG hepatocarcinoma cells. The iron oxide nanoparticles passed the gastrointestinal passage without dissolution and reached the intestine in the form of particles. Minor uptake was seen into Caco-2 cells but almost no transport to the basolateral site was detected for any of the tested particles. HepaRG cells showed higher particle uptake. Caco-2 cells showed no alterations in reactive oxygen species production, apoptosis, or mitochondrial membrane potential, whereas two particles induced apoptosis in HepaRG cells, and one altered mitochondrial membrane potential at non-cytotoxic concentrations. No correlation between physicochemical particle characteristics and cellular effects was observed, thus emphasizing the need for case-by-case assessment of iron oxide nanoparticles.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Potencial da Membrana Mitocondrial
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Nanopartículas Magnéticas de Óxido de Ferro
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Intestinos
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Neoplasias Hepáticas
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article