Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.

Diggins, Nicole L; Crawford, Lindsey B; Struthers, Hillary M; Hook, Lauren M; Landais, Igor; Skalsky, Rebecca L; Hancock, Meaghan H

Diggins, Nicole L; Crawford, Lindsey B; Struthers, Hillary M; Hook, Lauren M; Landais, Igor; Skalsky, Rebecca L; Hancock, Meaghan H.

Afiliação

Diggins NL; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Crawford LB; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Struthers HM; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Hook LM; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Landais I; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Skalsky RL; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Hancock MH; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA. hancocme@ohsu.edu.

Methods Mol Biol ; 2244: 301-342, 2021.

Article em En | MEDLINE | ID: mdl-33555594

Assuntos

Citomegalovirus/genética; Imunoprecipitação/métodos; MicroRNAs/análise; Regiões 3' não Traduzidas/genética; Northern Blotting/métodos; Expressão Gênica/genética; Regulação Viral da Expressão Gênica/genética; Humanos; MicroRNAs/genética; RNA Mensageiro/genética

Palavras-chave

Human cytomegalovirus (HCMV); Locked nucleic acids (LNA); Luciferase assay; Northern Blot; Photoactivatable ribonucleoside-enhanced cross-linking and Immunoprecipitation (PAR CLIP); RISC-immunoprecipitation (RISC-IP); Stem-loop real-time PCR; microRNA (miRNA)

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Citomegalovirus / MicroRNAs / Imunoprecipitação Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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