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DNA barcode analysis of the endangered green turtle (Chelonia mydas) in Mexico1.
Camacho-Sánchez, Fátima Yedith; Aguirre, A Alonso; Narváez-Zapata, José Alberto; Zavala-Norzagaray, Alan A; Ley-Quiñónez, Cesar P; Acosta-Sánchez, H Hugo; Rodriguez-González, Hervey; Delgado-Trejo, Carlos; Reyes-López, Miguel Angel.
Afiliação
  • Camacho-Sánchez FY; Conservation Medicine Lab, Centro de Biotecnología Genómica-Instituto Politécnico Nacional, Reynosa, Tamaulipas, Mexico.
  • Aguirre AA; Department of Environmental Science and Policy, George Mason University, Fairfax, Virginia, USA.
  • Narváez-Zapata JA; Department of Environmental Science and Policy, George Mason University, Fairfax, Virginia, USA.
  • Zavala-Norzagaray AA; Laboratorio de Biotecnología Industrial, Centro de Biotecnología Genómica-Instituto Politécnico Nacional, Reynosa, Tamaulipas, Mexico.
  • Ley-Quiñónez CP; Laboratorio de Vida Silvestre, Departamento de Medio Ambiente, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Unidad Sinaloa, Instituto Politécnico Nacional, Guasave, Sinaloa, Mexico.
  • Acosta-Sánchez HH; Laboratorio de Vida Silvestre, Departamento de Medio Ambiente, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Unidad Sinaloa, Instituto Politécnico Nacional, Guasave, Sinaloa, Mexico.
  • Rodriguez-González H; United Nations Development Programme-Comisión Nacional de Áreas Naturales Protegidas, Ciudad Victoria, Tamaulipas, Mexico.
  • Delgado-Trejo C; Laboratorio Nutrición Acuícola, Departamento de Acuacultura, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Unidad Sinaloa, Instituto Politécnico Nacional, Guasave, Sinaloa, Mexico.
  • Reyes-López MA; Departamento de Ecología Marina, Proyecto Tortuga Negra, Instituto de Investigaciones sobre los Recursos Naturales, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mexico.
Genome ; 64(9): 879-891, 2021 Sep.
Article em En | MEDLINE | ID: mdl-33555972
ABSTRACT
Technological and analytical advances to study evolutionary biology, ecology, and conservation of green turtles (Chelonia mydas) are realized through molecular approaches including DNA barcoding. We characterized the usefulness of COI DNA barcodes in green turtles in Mexico to better understand genetic divergence and other genetic parameters of this species. We analyzed 63 sequences, including 25 from green turtle field specimens collected from the Gulf of Mexico and from the Mexican Pacific and 38 already present in the Barcode of Life Data Systems (BOLD). A total of 13 haplotypes were identified with four novel haplotypes from the Pacific Ocean and three novel haplotypes from the Atlantic Ocean. Intraspecific distance values among COI gene sequences by two different models were 0.01, demonstrating that there is not a subdivision for green turtle species. Otherwise, the interspecific distance interval ranged from 0.07 to 0.13, supporting a clear subdivision among all sea turtle species. Haplotype and total nucleotide diversity values of the COI gene reflect a medium genetic diversity average. Green turtles of the Mexican Pacific showed common haplotypes to some Australian and Chinese turtles, but different from the haplotypes of the Mexican Atlantic. COI analysis revealed new haplotypes and confirmed that DNA barcodes were useful for evaluation of the population diversity of green turtles in Mexico.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tartarugas / Código de Barras de DNA Taxonômico Limite: Animals País como assunto: Mexico Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tartarugas / Código de Barras de DNA Taxonômico Limite: Animals País como assunto: Mexico Idioma: En Ano de publicação: 2021 Tipo de documento: Article