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Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags.
Barros, Isabella Oliveira; Sousa, Rejane Santos; Tavares, Marcondes Dias; Rêgo, Renato Otaviano; Firmino, Paulo Ricardo; Souza, Francisco Jocelho Alexandre; Abrantes, Maria Rociene; Minervino, Antonio Humberto Hamad; Araújo, Carolina Akiko Sato Cabral; Ortolani, Enrico Lippi; Barrêto Júnior, Raimundo Alves.
Afiliação
  • Barros IO; Department of Veterinary Science, Federal University of Paraíba, Rodovia PB 079, Km 12, Areia 58397-000, Brazil.
  • Sousa RS; Institute of Studies of the Humid Tropic, Federal University of the South and Southeast of Pará, Rua Alberto Santos Dumont, s/n, Xinguara 68557-335, Brazil.
  • Tavares MD; Department of Animal Science, Federal Rural University of the Semi-Arid, Av. Francisco Mota, Mossoró 59625-900, Brazil.
  • Rêgo RO; Department of Animal Science, Federal Rural University of the Semi-Arid, Av. Francisco Mota, Mossoró 59625-900, Brazil.
  • Firmino PR; Department of Animal Science, Federal Rural University of the Semi-Arid, Av. Francisco Mota, Mossoró 59625-900, Brazil.
  • Souza FJA; Department of Animal Science, Federal Rural University of the Semi-Arid, Av. Francisco Mota, Mossoró 59625-900, Brazil.
  • Abrantes MR; Department of Animal Science, Federal Rural University of the Semi-Arid, Av. Francisco Mota, Mossoró 59625-900, Brazil.
  • Minervino AHH; Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz No Number, Santarém 68040-080, Brazil.
  • Araújo CASC; Department of Veterinary Medicine, Federal Rural University of Pernambuco, Rua Dom Manuel Medeiros, no number, Recife 52171-900, Brazil.
  • Ortolani EL; School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Dr. Orlando Paiva, 97, São Paulo 05508-270, Brazil.
  • Barrêto Júnior RA; Department of Animal Science, Federal Rural University of the Semi-Arid, Av. Francisco Mota, Mossoró 59625-900, Brazil.
Biology (Basel) ; 10(2)2021 Feb 08.
Article em En | MEDLINE | ID: mdl-33567685
Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article