Precise identification of an RNA methyltransferase's substrate modification site.
Chem Commun (Camb)
; 57(20): 2499-2502, 2021 Mar 09.
Article
em En
| MEDLINE
| ID: mdl-33586715
ABSTRACT
Here we report a simple and nonradioactive biochemical assay which is capable of accurately determining the substrate methylation sites of human RNA N6-methyladenosine methyltransferases METTL3/METTL14 and METTL16. This method employs enzyme-assisted chemical labelling of a specific base in an RNA substrate with the assistance of an allyl-substituted methyltransferase cofactor, and enables precise identification of the labelling site by a mutation signal from standard nucleic acid sequencing. Our method provides a platform to investigate the enzymatic methylations of long and structurally complex RNA substrates, and facilitates the discovery of new methyltransferases.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Metiltransferases
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article