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Decoding the protein composition of whole nucleosomes with Nuc-MS.
Schachner, Luis F; Jooß, Kevin; Morgan, Marc A; Piunti, Andrea; Meiners, Matthew J; Kafader, Jared O; Lee, Alexander S; Iwanaszko, Marta; Cheek, Marcus A; Burg, Jonathan M; Howard, Sarah A; Keogh, Michael-Christopher; Shilatifard, Ali; Kelleher, Neil L.
Afiliação
  • Schachner LF; Department of Chemistry, Northwestern University, Evanston, IL, USA.
  • Jooß K; The Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.
  • Morgan MA; Department of Chemistry, Northwestern University, Evanston, IL, USA.
  • Piunti A; The Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
  • Meiners MJ; Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Kafader JO; Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Lee AS; Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Iwanaszko M; Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Cheek MA; EpiCypher, Inc., Research Triangle Park, Durham, NC, USA.
  • Burg JM; Department of Chemistry, Northwestern University, Evanston, IL, USA.
  • Howard SA; The Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
  • Keogh MC; The Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.
  • Shilatifard A; The Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
  • Kelleher NL; Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Nat Methods ; 18(3): 303-308, 2021 03.
Article em En | MEDLINE | ID: mdl-33589837
ABSTRACT
Current proteomic approaches disassemble and digest nucleosome particles, blurring readouts of the 'histone code'. To preserve nucleosome-level information, we developed Nuc-MS, which displays the landscape of histone variants and their post-translational modifications (PTMs) in a single mass spectrum. Combined with immunoprecipitation, Nuc-MS quantified nucleosome co-occupancy of histone H3.3 with variant H2A.Z (sixfold over bulk) and the co-occurrence of oncogenic H3.3K27M with euchromatic marks (for example, a >15-fold enrichment of dimethylated H3K79me2). Nuc-MS is highly concordant with chromatin immunoprecipitation-sequencing (ChIP-seq) and offers a new readout of nucleosome-level biology.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Histonas / Nucleossomos / Espectrometria de Massas por Ionização por Electrospray / Proteômica Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
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XML
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Histonas / Nucleossomos / Espectrometria de Massas por Ionização por Electrospray / Proteômica Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article