In Vitro Induction of Teleost PGCs.
Methods Mol Biol
; 2218: 75-83, 2021.
Article
em En
| MEDLINE
| ID: mdl-33606224
ABSTRACT
Primordial germ cells (PGCs) are unique cells in an embryo. These cells contain all genetic information and therefore represent the best source to store maternal and paternal genomes until embryo cryopreservation is achieved. However, the number of these cells in an embryo is very low limiting their potential application in cryopreservation and surrogate production. However, it was assumed that the induction of fish PGCs in vitro is not possible because in vivo they inherit germ plasm. In this chapter, we describe a successful differentiation protocol explaining the crucial factors and steps for in vitro PGC generation.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Germinativas
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article