The extrachromosomal elements of the Naegleria genus: How little we know.
Plasmid
; 115: 102567, 2021 05.
Article
em En
| MEDLINE
| ID: mdl-33617907
ABSTRACT
There are currently 47 characterized species in the Naegleria genus of free-living amoebae. Each amoeba has thousands of extrachromosomal elements that are closed circular structures comprised of a single ribosomal DNA (rDNA) copy and a large non-rDNA sequence. Despite the presence of putative open reading frames and introns, ribosomal RNA is the only established transcript. A single origin of DNA replication (ori) has been mapped within the non-rDNA sequence for one species (N. gruberi), a finding that strongly indicates that these episomes replicate independently of the cell's chromosomal DNA component. This article reviews that which has been published about these interesting DNA elements and by analyzing available sequence data, discusses the possibility that different phylogenetically related clusters of Naegleria species individually conserve ori structures and suggests where the rRNA promoter and termination sites may be located.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Naegleria
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article