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Genome-scale deconvolution of RNA structure ensembles.
Morandi, Edoardo; Manfredonia, Ilaria; Simon, Lisa M; Anselmi, Francesca; van Hemert, Martijn J; Oliviero, Salvatore; Incarnato, Danny.
Afiliação
  • Morandi E; Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, the Netherlands.
  • Manfredonia I; Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Torino, Italy.
  • Simon LM; Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, the Netherlands.
  • Anselmi F; Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Torino, Italy.
  • van Hemert MJ; Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Torino, Italy.
  • Oliviero S; Department of Medical Microbiology, Molecular Virology Laboratory, Leiden University Medical Center, Leiden, the Netherlands.
  • Incarnato D; Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Torino, Italy. salvatore.oliviero@unito.it.
Nat Methods ; 18(3): 249-252, 2021 03.
Article em En | MEDLINE | ID: mdl-33619392
RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3' untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Algoritmos / RNA Viral / Genoma Viral / SARS-CoV-2 / Conformação de Ácido Nucleico Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Algoritmos / RNA Viral / Genoma Viral / SARS-CoV-2 / Conformação de Ácido Nucleico Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article