Comparison of fiber size and phenotypic gene expression in muscles of dystrophic C57BL/6J DY2J/DY2J mice.
Neurosci Lett
; 85(3): 370-4, 1988 Mar 10.
Article
em En
| MEDLINE
| ID: mdl-3362425
Considerable evidence has shown a correlation between fiber size and degree of necrosis in dystrophic muscles of hamster, X-linked muscular dystrophy (MDX) mice and humans. It has been proposed that small-caliber fibers have an immunity to the phenotypic expression of the dystrophic gene(s). The results from the present study show a discordance between fiber size and necrosis in dystrophic muscles of C57BL/6J dy2J/dy2J mice. Extensor carpi radialis longus and brevis muscles (ECRL and ECRB respectively) were compared in normal and dystrophic 2-week, 4-week and 12-month animals by measuring the mean cross-sectional area of type II fibers, determination of relative proportions of types IIA and IIB fibers and calculation of percentage of fibers exhibiting centronucleation in an entire cross-section of muscle (stained for haematoxylin and eosin or ATPase). The ECRL and ECRB muscles were found to have identical sizes of fiber at each of the 3 ages studied and similar proportions of fiber types, yet the former muscle developed and retained significantly more necrosis (manifest as centronucleation) than the latter.
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Base de dados:
MEDLINE
Assunto principal:
Fenótipo
/
Regulação da Expressão Gênica
/
Músculos
/
Distrofia Muscular Animal
Limite:
Animals
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article