[Effects of Apelin-13 on barrier injury of human umbilical vein endothelial cells induced by LPS].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 36(5): 390-393, 2020 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-33629549
ABSTRACT
Objective:
To investigate the effects of Apelin-13 on barrier function injury of human umbilical vein endothelial cells (HUVECs) induced by LPS.Methods:
The HUVECs cultured in vitro were divided into 4 groups Control group, LPS group, Apelin-13+LPS group, Apelin-13 group. HUVECs were treated by 5 µg/ml LPS for 24 h to replicate the model with endothelial barrier impaired. Apelin-13 at the concentration of 1 µmol/L was given 30 min before LPS treatment. The cell viabillity of HUVECs was measured by CCK-8 assay. Protein expressions of VE-cadherin and F-actin were measured by Western blot and immunofluorescence. Nuclear factor κB p65(NF-κB p65) was detected by immunofluorescence.Results:
Compared with the control group, the cell viabillity of HUVECs and protein expression of VE-cadherin were decreased by LPS, but the protein expression of F-actin and activation of NF-κB p65 were increased by LPS. These effects were attenuated by Apelin-13 administration.Conclusion:
Apelin-13 ameliorates LPS-induced barrier function injury of HUVECs, which may be related to the inhibition of inflammation.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Lipopolissacarídeos
/
NF-kappa B
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2020
Tipo de documento:
Article