Promises and Pitfalls of Parasite Patch-clamp.
Trends Parasitol
; 37(5): 414-429, 2021 05.
Article
em En
| MEDLINE
| ID: mdl-33640269
ABSTRACT
Protozoan parasites acquire essential ions, nutrients, and other solutes from their insect and vertebrate hosts by transmembrane uptake. For intracellular stages, these solutes must cross additional membranous barriers. At each step, ion channels and transporters mediate not only this uptake but also the removal of waste products. These transport proteins are best isolated and studied with patch-clamp, but these methods remain accessible to only a few parasitologists due to specialized instrumentation and the required training in both theory and practice. Here, we provide an overview of patch-clamp, describing the advantages and limitations of the technology and highlighting issues that may lead to incorrect conclusions. We aim to help non-experts understand and critically assess patch-clamp data in basic research studies.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Parasitos
/
Parasitologia
/
Técnicas de Patch-Clamp
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article