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Functionalized graphene oxide in situ initiated ring-opening polymerization for highly sensitive sensing of cytokeratin-19 fragment.
Liu, Yanju; Hao, Lulu; Wang, Wenbin; Yang, Huaixia; Si, Fuchun; Kong, Jinming.
Afiliação
  • Liu Y; Pharmacy College, Henan University of Chinese Medicine, Zhengzhou, 450046, People's Republic of China.
  • Hao L; Pharmacy College, Henan University of Chinese Medicine, Zhengzhou, 450046, People's Republic of China.
  • Wang W; Henan Key Laboratory of TCM Syndrome and Prescription in Signaling, Henan International Joint Laboratory of TCM Syndrome and Prescription in Signaling, Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, People's Republic of China.
  • Yang H; Pharmacy College, Henan University of Chinese Medicine, Zhengzhou, 450046, People's Republic of China. yhx998@hactcm.edu.cn.
  • Si F; Henan Key Laboratory of TCM Syndrome and Prescription in Signaling, Henan International Joint Laboratory of TCM Syndrome and Prescription in Signaling, Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, People's Republic of China. sifc2000@hotmail.com.
  • Kong J; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, People's Republic of China. j.kong@njust.edu.cn.
Mikrochim Acta ; 188(4): 123, 2021 03 12.
Article em En | MEDLINE | ID: mdl-33712913
Improving the sensitivity of detection is crucial to monitor biomarker, assess toxicity, and track therapeutic agent. Herein, a sensitivity-improved immunosensor is reported for the first time via functionalized graphene oxide (GO) and a "grafting-to" ring-opening polymerization (ROP) dual signal amplification strategy. Through the ROP reaction using 2-[(4-ferrocenylbutoxy)methyl] oxirane (FcEpo) as the monomer, lots of electroactive tags are linked in situ from multiple initiation sites on the GO surface modified with ethanol amine (GO-ETA), thereby achieving high sensitivity even in the case of trace amounts of tumor markers. The utmost important factor for achieving this high sensitivity is to select functionalized GO as the initiator that contains a large number of repeated hydroxyl functional groups so as to trigger additional ROP reaction. Under the optimal conditions, the high sensitivity and applicability is demonstrated by the use of GO-ETA-mediated ROP-based immunosensor to detect non-small cell lung cancer (NSCLC)-specific biomarker down to 72.58 ag/mL (equivalent to ~6 molecules in a 5 µL sample). Furthermore, the satisfactory results for the determination of biomarkers in clinical serum samples highlighted that this immunosensor holds a huge potential in practical clinical application. This work described an electrochemical immunosensor for ultrasensitive detection of CYFRA 21-1 via the functionalized graphene oxide (GO) and a "grafting-to" ring-opening polymerization (ROP) dual signal amplification strategy, which hold the merits of high sensitivity, applicability, selectivity, efficiency, easy operation and environmental friendliness.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fragmentos de Peptídeos / Biomarcadores Tumorais / Queratina-19 / Grafite / Antígenos de Neoplasias Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fragmentos de Peptídeos / Biomarcadores Tumorais / Queratina-19 / Grafite / Antígenos de Neoplasias Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article