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A multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study.
Radzieta, Michael; Sadeghpour-Heravi, Fatemah; Peters, Timothy J; Hu, Honghua; Vickery, Karen; Jeffries, Thomas; Dickson, Hugh G; Schwarzer, Saskia; Jensen, Slade O; Malone, Matthew.
Afiliação
  • Radzieta M; South Western Sydney Limb Preservation and Wound Research, South Western Sydney LHD, Sydney, Australia. M.Radzieta@westernsydney.edu.au.
  • Sadeghpour-Heravi F; Infectious Diseases and Microbiology, School of Medicine, Western Sydney University, Sydney, Australia. M.Radzieta@westernsydney.edu.au.
  • Peters TJ; Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia.
  • Hu H; Immunogenomics Laboratory, Immunology Division, Garvan Institute of Medical Research, Sydney, Australia.
  • Vickery K; Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia.
  • Jeffries T; Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia.
  • Dickson HG; School of Science and Health, Western Sydney University, Sydney, Australia.
  • Schwarzer S; South Western Sydney Limb Preservation and Wound Research, South Western Sydney LHD, Sydney, Australia.
  • Jensen SO; South Western Clinical School, University of New South Wales, Sydney, Australia.
  • Malone M; South Western Sydney Limb Preservation and Wound Research, South Western Sydney LHD, Sydney, Australia.
NPJ Biofilms Microbiomes ; 7(1): 29, 2021 03 22.
Article em En | MEDLINE | ID: mdl-33753735
Diabetic foot infections (DFIs) are a major cause of hospitalization and can lead to lower extremity amputation. In this pilot study, we used a multiomics approach to explore the host-microbe complex within DFIs. We observed minimal differences in the overall microbial composition between PEDIS infection severities, however Staphylococcus aureus and Streptococcus genera were abundant and highly active in most mild to moderate DFIs. Further, we identified the significant enrichment of several virulence factors associated with infection pathogenicity belonging to both Staphylococcus aureus and Streptococcus. In severe DFIs, patients demonstrated a greater microbial diversity and differential gene expression demonstrated the enrichment of multispecies virulence genes suggestive of a complex polymicrobial infection. The host response in patients with severe DFIs was also significantly different as compared to mild to moderate DFIs. This was attributed to the enrichment of host genes associated with inflammation, acute phase response, cell stress and broad immune-related responses, while those associated with wound healing and myogenesis were significantly depleted.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias / Pé Diabético / Perfilação da Expressão Gênica / Fatores de Virulência / Metagenômica / Coinfecção Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans / Male Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias / Pé Diabético / Perfilação da Expressão Gênica / Fatores de Virulência / Metagenômica / Coinfecção Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans / Male Idioma: En Ano de publicação: 2021 Tipo de documento: Article