Control of Triplet Blinking Using Cyclooctatetraene to Access the Dynamics of Biomolecules at the Single-Molecule Level.
Angew Chem Int Ed Engl
; 60(23): 12941-12948, 2021 06 01.
Article
em En
| MEDLINE
| ID: mdl-33783937
ABSTRACT
To explore the dynamics of biomolecules, tracing the kinetics of photo-induced chemical reactions via the triplet excited state (T1 ) of probe molecules offers a timescale that is about 106 times wider than via the singlet excited state (S1 ). Using cyclooctatetraene (COT) as a triplet energy acceptor and at the same time as a photostabilizer, the triplet-triplet energy transfer (TTET) kinetics governed by oligonucleotide (oligo) dynamics were studied at the single-molecule level by measuring fluorescence blinking. TTET kinetics measurement allowed us to access the length- and sequence-dependent dynamics of oligos and realize the single-molecule detection of a model microRNA biomarker. In sharp contrast to the singlet-singlet Förster resonance energy transfer (FRET) that occurs in the 1-10â
nm range, TTET requires a Van der Waals contact. The present method is thus a complementary method to FRET and provides direct information on biomolecular dynamics on the µs to ms timescale.
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Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Ciclo-Octanos
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Imagem Individual de Molécula
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article