Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay.
Methods Mol Biol
; 2267: 57-71, 2021.
Article
em En
| MEDLINE
| ID: mdl-33786784
ABSTRACT
DNA replication is a fundamental process of life. Any perturbation of this process by endogenous or exogenous factors impacts on genomic stability and thereby on carcinogenesis. More recently, the replication machinery has been discovered as an interesting target for cancer therapeutic strategies. Given its high biological and clinical relevance, technologies for the analysis of DNA replication have attracted major attention. The so-called DNA fiber spreading technique is a powerful tool to directly monitor various aspects of the replication process by sequential incorporation of halogenated nucleotide analogs which later can be fluorescently stained and analyzed. This chapter outlines the use of the DNA fiber spreading technique for the analysis of replication dynamics and replication structures.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Replicação do DNA
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Imagem Individual de Molécula
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article