Bacterial cyclic diguanylate signaling networks sense temperature.
Nat Commun
; 12(1): 1986, 2021 03 31.
Article
em En
| MEDLINE
| ID: mdl-33790266
Many bacteria use the second messenger cyclic diguanylate (c-di-GMP) to control motility, biofilm production and virulence. Here, we identify a thermosensory diguanylate cyclase (TdcA) that modulates temperature-dependent motility, biofilm development and virulence in the opportunistic pathogen Pseudomonas aeruginosa. TdcA synthesizes c-di-GMP with catalytic rates that increase more than a hundred-fold over a ten-degree Celsius change. Analyses using protein chimeras indicate that heat-sensing is mediated by a thermosensitive Per-Arnt-SIM (PAS) domain. TdcA homologs are widespread in sequence databases, and a distantly related, heterologously expressed homolog from the Betaproteobacteria order Gallionellales also displayed thermosensitive diguanylate cyclase activity. We propose, therefore, that thermotransduction is a conserved function of c-di-GMP signaling networks, and that thermosensitive catalysis of a second messenger constitutes a mechanism for thermal sensing in bacteria.
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Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
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Proteínas de Bactérias
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Sistemas do Segundo Mensageiro
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Transdução de Sinais
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GMP Cíclico
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Fósforo-Oxigênio Liases
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Proteínas de Escherichia coli
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article