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Skeletal muscle fibers play a functional role in host defense during sepsis in mice.
Laitano, Orlando; Robinson, Gerard P; Murray, Kevin O; Garcia, Christian K; Mattingly, Alex J; Morse, Deborah; King, Michelle A; Iwaniec, John D; Alzahrani, Jamal M; Clanton, Thomas L.
Afiliação
  • Laitano O; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
  • Robinson GP; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
  • Murray KO; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
  • Garcia CK; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
  • Mattingly AJ; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
  • Morse D; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
  • King MA; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
  • Iwaniec JD; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
  • Alzahrani JM; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
  • Clanton TL; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA. tclanton@hhp.ufl.edu.
Sci Rep ; 11(1): 7316, 2021 04 01.
Article em En | MEDLINE | ID: mdl-33795743
Skeletal muscles secrete a wide variety of immunologically active cytokines, but the functional significance of this response to in vivo innate immunity is not understood. We addressed this by knocking out the toll receptor adapter protein, Myd88, only in skeletal muscle fibers (skmMyd88KO), and followed male and female mice at 6 and 12 h after peritoneal injection of cecal slurry (CS), a model of polymicrobial sepsis. Because of a previously identified increase in mortality to CS injection, males received ~ 30% lower dose. At 12 h, skmMyd88KO caused significant reductions in a wide variety of pro- and anti-inflammatory plasma cytokines, e.g. TNFα, IL-1ß and IL-10, compared to strain-matched controls in both males and females. Similar reductions were observed at 6 h in females. SkmMyd88KO led to ~ 40-50% elevations in peritoneal neutrophils at 6 and 12 h post CS in females. At 12 h post CS, skmMyd88KO increased peritoneal monocytes/macrophages and decreased %eosinophils and %basophils in females. SkmMyd88KO also led to significantly higher rates of mortality in female mice but not in males. In conclusion, the results suggest that skeletal muscle Myd88-dependent signal transduction can play functionally important role in normal whole body, innate immune inflammatory responses to peritoneal sepsis.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Sepse / Músculo Esquelético / Fator 88 de Diferenciação Mieloide Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Sepse / Músculo Esquelético / Fator 88 de Diferenciação Mieloide Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article