Making the most of high-dimensional cytometry data.
Immunol Cell Biol
; 99(7): 680-696, 2021 08.
Article
em En
| MEDLINE
| ID: mdl-33797774
ABSTRACT
High-dimensional cytometry represents an exciting new era of immunology research, enabling the discovery of new cells and prediction of patient responses to therapy. A plethora of analysis and visualization tools and programs are now available for both new and experienced users; however, the transition from low- to high-dimensional cytometry requires a change in the way users think about experimental design and data analysis. Data from high-dimensional cytometry experiments are often underutilized, because of both the size of the data and the number of possible combinations of markers, as well as to a lack of understanding of the processes required to generate meaningful data. In this article, we explain the concepts behind designing high-dimensional cytometry experiments and provide considerations for new and experienced users to design and carry out high-dimensional experiments to maximize quality data collection.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Citometria de Fluxo
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article