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Genome Analysis of Endotrypanum and Porcisia spp., Closest Phylogenetic Relatives of Leishmania, Highlights the Role of Amastins in Shaping Pathogenicity.
Albanaz, Amanda T S; Gerasimov, Evgeny S; Shaw, Jeffrey J; Sádlová, Jovana; Lukes, Julius; Volf, Petr; Opperdoes, Fred R; Kostygov, Alexei Y; Butenko, Anzhelika; Yurchenko, Vyacheslav.
Afiliação
  • Albanaz ATS; Life Science Research Centre, Faculty of Science, University of Ostrava, 71000 Ostrava, Czech Republic.
  • Gerasimov ES; Faculty of Biology, M. V. Lomonosov Moscow State University, 119991 Moscow, Russia.
  • Shaw JJ; Biomedical Institute, São Paulo University, São Paulo 05508, Brazil.
  • Sádlová J; Department of Parasitology, Faculty of Science, Charles University, 12844 Prague, Czech Republic.
  • Lukes J; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 Ceské Budejovice, Czech Republic.
  • Volf P; Faculty of Science, University of South Bohemia, 37005 Ceské Budejovice, Czech Republic.
  • Opperdoes FR; Department of Parasitology, Faculty of Science, Charles University, 12844 Prague, Czech Republic.
  • Kostygov AY; de Duve Institute, Université Catholique de Louvain, 1200 Brussels, Belgium.
  • Butenko A; Life Science Research Centre, Faculty of Science, University of Ostrava, 71000 Ostrava, Czech Republic.
  • Yurchenko V; Zoological Institute of the Russian Academy of Sciences, 199034 St. Petersburg, Russia.
Genes (Basel) ; 12(3)2021 03 20.
Article em En | MEDLINE | ID: mdl-33804709
While numerous genomes of Leishmania spp. have been sequenced and analyzed, an understanding of the evolutionary history of these organisms remains limited due to the unavailability of the sequence data for their closest known relatives, Endotrypanum and Porcisia spp., infecting sloths and porcupines. We have sequenced and analyzed genomes of three members of this clade in order to fill this gap. Their comparative analyses revealed only minute differences from Leishmaniamajor genome in terms of metabolic capacities. We also documented that the number of genes under positive selection on the Endotrypanum/Porcisia branch is rather small, with the flagellum-related group of genes being over-represented. Most significantly, the analysis of gene family evolution revealed a substantially reduced repertoire of surface proteins, such as amastins and biopterin transporters BT1 in the Endotrypanum/Porcisia species when compared to amastigote-dwelling Leishmania. This reduction was especially pronounced for δ-amastins, a subfamily of cell surface proteins crucial in the propagation of Leishmania amastigotes inside vertebrate macrophages and, apparently, dispensable for Endotrypanum/Porcisia, which do not infect such cells.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Trypanosomatina / Sequenciamento Completo do Genoma / Proteínas de Membrana Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Trypanosomatina / Sequenciamento Completo do Genoma / Proteínas de Membrana Idioma: En Ano de publicação: 2021 Tipo de documento: Article