Manipulation of the Regulatory Genes ppsR and prrA in Rhodobacter sphaeroides Enhances Lycopene Production.
J Agric Food Chem
; 69(14): 4134-4143, 2021 Apr 14.
Article
em En
| MEDLINE
| ID: mdl-33813825
ABSTRACT
Rhodobacter sphaeroides is a non-sulfur purple bacterium with great metabolic versatility, capable of producing a variety of valuable compounds that include carotenoids and CoQ10. In order to enhance lycopene production, we deleted the photosynthetic gene cluster repressor ppsR from a lycopene-producing Rb. sphaeroides strain (RL1) constructed in a previous study to break the control of carotenoid synthesis by the oxygen level. Also, lycopene production was further increased by overexpression of the activator prrA. The superior lycopene producer DppsR/OprrA thus obtained had a high growth rate and a lycopene production of 150.15 mg/L with a yield of 21.45 mg/g dry cell weight (DCW) under high oxygen conditions; these values were ≥6.85-fold higher than those of RL1 (19.13 mg/L; 3.32 mg/g DCW). Our findings indicate that elimination of oxygen repression led to more efficient lycopene production by DppsR/OprrA and that its increased productivity under high oxygen conditions makes it a potentially useful strain for industrial-scale lycopene production.
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MEDLINE
Assunto principal:
Rhodobacter sphaeroides
Idioma:
En
Ano de publicação:
2021
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Article