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Perinatal exposure to di-(2-ethylhexyl) phthalate induces hepatic lipid accumulation mediated by diacylglycerol acyltransferase 1.
An, S J; Lee, E J; Jeong, S-H; Hong, Y-P; Ahn, S; Yang, Y-J.
Afiliação
  • An SJ; Department of Neurology, Catholic Kwandong University International St Mary's Hospital, Incheon, Republic of Korea.
  • Lee EJ; These authors are equally contributed to this work.
  • Jeong SH; Institute for Catholic Integrative Medicine, Incheon St Mary's Hospital, College of Medicine, The Catholic University of Korea, Incheon, Republic of Korea.
  • Hong YP; These authors are equally contributed to this work.
  • Ahn S; Division of Gastroenterology, Department of Internal Medicine, Catholic Kwandong University International St Mary's Hospital, Incheon, Republic of Korea.
  • Yang YJ; Department of Preventive Medicine, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
Hum Exp Toxicol ; 40(10): 1698-1709, 2021 Oct.
Article em En | MEDLINE | ID: mdl-33832334
ABSTRACT

INTRODUCTION:

Di-(2-ethylhexyl) phthalate (DEHP) is a commonly used plasticizer in consumer products and medical devices. It is also suspected to exacerbate the development of fatty liver. However, the mechanisms underlying excessive lipid synthesis and its deposition in the liver are yet to be identified. This study was aimed to evaluate the molecular mechanisms of hepatic lipid accumulation in adult male offspring after perinatal exposure to DEHP.

METHOD:

Corn oil and DEHP (0.75 mg/kg/day) were administered once per day to dam from gestation day 6 to postnatal day (PND) 21 by oral gavage. After the weaning period, DEHP treated male pups were categorized into early life stage- and lifelong period group. Male rats both control and early life stage group administered corn oil, and lifelong period group administered DEHP from PND 22 to 70. Histological examination and triglyceride (TG) levels in the liver were analyzed. Expressions of transcription factors associated with lipid accumulation in the liver were analyzed.

RESULTS:

Both early life stage- and lifelong period group, hepatic TG levels, and mRNA and protein expression of diacylglycerol acyltransferase 1 (DGAT1) were significantly higher than control (TG all p < 0.05, mRNA & protein p < 0.05 and p < 0.001, respectively). The average body weight from PND 35 to 63, and mRNA and protein expression of sterol regulatory element binding protein 1c in lifelong period group were significantly lower than control (all p < 0.05); however, alanine transaminase were significantly higher than control (p < 0.01).

CONCLUSION:

Perinatal exposure to DEHP may induce the hepatic lipid accumulation through up-regulation of DGAT1 expression.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dietilexilftalato / Hepatopatia Gordurosa não Alcoólica / Fígado Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dietilexilftalato / Hepatopatia Gordurosa não Alcoólica / Fígado Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article